Journal of Medical Cases, ISSN 1923-4155 print, 1923-4163 online, Open Access
Article copyright, the authors; Journal compilation copyright, J Med Cases and Elmer Press Inc
Journal website http://www.journalmc.org

Case Report

Volume 7, Number 1, January 2016, pages 7-12

Cytogenetic Findings of an Undifferentiated Testicular Sarcoma

Figures

Figure 1.
Figure 1. Gross tumor was surrounded by tunica albuginea. Cut sections showed various parts including yellowish-white solid part, myxomatous part, hemorrhage and necrotic part.
Figure 2.
Figure 2. Tumor cells had irregularly shaped bundles of pleomorphic spindle or round cells, oval shaped, and irregularly shaped hyperchromatic nuclei with coarsely granular chromatin particles. Most of these cells had pale staining cytoplasm (H&E).
Figure 3.
Figure 3. Isolated small number of cells had large eosinophilic or clear cytoplasm with round nuclei (H&E).
Figure 4.
Figure 4. A few large cells that have eosinophilic or clear cytoplasm were stained by MSA.
Figure 5.
Figure 5. FISH displayed orange signals for the chromosome 12 centromeric prove (orange arrow) and green signals for 12p (green arrow).

Table

Table 1. Antibodies and Technique Used for Immunohistochemical Staining
 
AntibodyCorporationCloneDilutionEpitope retrieval
*P: enzyme digestion (proteinase K) incubated at room temperature for 5 min. **RTU: ready to use. ***HIER: heat-induced epitope retrieval (0.01 M citrate buffer pH 6.0) incubated at 95 °C for 40 min.
CytokeratinDAKOAE1/AE31:50P*
DesminNichireiD33RTU**HIER***
NSEDAKOBBS/NC/VI-H14RTUHIER
α-SMADAKO1A41:100None
MIC-2 (CD99)DAKO1.20E08RTUHIER
EMADAKOE291:100None
CD30DAKOBer-H2RTUHIER
MyoglobinDAKOPolyclonalRTUNone
S-100NichireiPolyclonalRTUNone
CK34βE12Novocastra34βE121:100HIER
AFPNichireiPolyclonalRTUNone
PLAPDAKO8A91:100HIER
HCG-βNichireiC6405RTUP
CalretininNichireiSP12RTUHIER
Inhibin-αSerotecR11:50HIER
4-OctJapan Tanner Co.MRQ-101:100HIER
VimentinDAKOV91:100HIER
c-kitNichireiPolyclonalRTUHIER
MSADAKOHHF351:100None